Glyphosate is often tank-mixed with auxinic herbicide 2,4-D for grass and broadleaf weed control. In this paper, the authors examined the possible interaction of 2,4-D and glyphosate in barnyard grass, Echinochloa colona. The results showed that 2,4-D antagonizes glyphosate remarkably in glyphosate resistant populations but only marginally in susceptible populations. This antagonism is related to reduced glyphosate uptake and (to a lesser extent) translocation. As 2,4-D has multiple, unpredictable effects on other herbicides, care must be taken when tank-mixing herbicides with 2,4-D.
The auxinic herbicide 2,4-D amine is known, in vitro, as a cytochrome P450 inducer. The current study uses 2,4-D pre-treatment, at the whole plant level, to study mechanism(s) of non-target site based herbicide resistance to the ACCase-inhibiting herbicide diclofop-methyl in Lolium rigidum.