This study shows that the resistance mechanism endowing pyroxasulfone resistance in this Lolium rigidum population is enhanced rates of pyroxasulfone metabolism. This enhanced rate of pyroxasulfone metabolism is associated with over-expression of two glutathione transferases. Thus, the resistance mechanism in this Lolium population of enhanced rates of pyroxasulfone metabolism suggests GST-catalysed increased rates of glutathione conjugation. More work is required for definitive evidence of specific responsible GSTs.